X光螢光顯微鏡 X-ray fluorescence microscopy imaging

X光源螢光顯微鏡 X-ray fluorescence microscopy imaging

這是很特別的螢光顯微鏡，以 X 光為光源，可以看出植物體元素分佈

a.為大麥粒縱切面，可以看到鐵、錳、鋅元素分佈

b.菥蓂屬幼苗圖像，為十字花科下的一個屬，證實可以有效吸收重金屬。

c.d. 鳳尾蕨科植物，可以有效吸收砷清除環境中的重金屬，淨化水源，可以看到砷在葉片中的分佈。

e. 小麥X光螢光顯微鏡(XFM)圖像

Figure 1. Distribution of nutrients in plant tissues examined using XFM. (a) A tri-color image showing Fe, Mn, and Zn distribution in a longitudinal section (70 μm thick) of a barley grain. The original scan was 2320 × 880 pixels (ca. 2 megapixels) with a pixel transit time of ca. 0.6 ms resulting in a scan duration of ca. 20 min (see Lombi et al. (2011c)). (b) Image from XFM analyses of a scan of a living seedling of Noccaea caerulescens (a hyperaccumulator) showing Zn (green), K (red), and Ca (blue). Data from A. van der Ent (unpublished). (c) and (d) Images from XFM analyses of scans of a leaflet of Pteris vittata (an As hyperaccumulator) showing K (green), As (red), and Ca (blue). Data from R.V. Tappero (unpublished). (e) An initial attempt using XFM to rapidly scan whole and intact wheat seeds (cv. Shield). The transit per 20-μm pixel was 15 mm/s velocity, with the entire scan duration being ca. 8 min. Data from E. Lombi (unpublished).

資料來源: 

Plant Physiology Preview. Published on August 14, 2018, as DOI:10.1104/pp.18.00759