ZmMTOPVIB Enables DNA Double-Strand Break Formation and Bipolar Spindle Assembly during Maize Meiosis

ZmMTOPVIB Enables DNA Double-Strand Break Formation and Bipolar Spindle Assembly during Maize Meiosis

Figure 2.

Sterile phenotypes in ZmmtoVIB-1 mutant. A, Tassels of the wild type and ZmmtopVIB-1 at flowering stage. Scale bar = 10 cm. B, Anthers from glumes of spikelets removed from the wild type and ZmmtopVIB-1 at the flowering stage. Scale bar = 5 mm. C, Mature pollen grains from the wild type stained with Alexander solution. Scale bar = 100 μm. D, Sterile pollen grains from ZmmtopVIB-1 stained with Alexander solution. Scale bar = 100 μm. E, Harvested ears of the wild type and ZmmtopVIB-1 pollinated with wild-type pollen grains. Scale bar = 5 cm.

Figure 8.

Meiotic spindle assembly process in the wild type (A–D) and ZmmtopVIB-1 (E–L) in diakinesis (A, E, and I), metaphase I (B, F, and J), anaphase I (C, G, and K), and telophase I (D, H, and L) stages. Chromosomes were marked with DAPI (blue) and microtubules with α-tubulin antibody (green). Red arrows point out the abnormal spindles and equatorial plates. Scale bars = 10 μm.

資料來源:

http://www.plantphysiol.org/content/184/4/1811